2021

Celldeath: A tool for detection of cell death in transmitted light microscopy images by deep learning-based visual recognition

Alejandro La Greca, Nelba Pérez, Sheila Castañeda, Paula Melania Milone, María Agustina Scarafía, Alan Miqueas Möbbs, Ariel Waisman, Lucía Natalia Moro, Gustavo Sevlever, Carlos Luzzani, Santiago Gabriel Miriuka

Plos One 2021


miR-302 family, miR-145 and miR-296 temporal expression profile along the cell cycle of human pluripotent stem cells

Rodríguez-Varela MS, Mucci S, Videla-Richardson GA, Isaja L, Sevlever GE, Scassa ME, Romorini L.

Gene Expr Patterns 2021


Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT

CE Aban, Antonella Lombardi, Gabriel Neiman, Maria Celeste Biani, Alejandro La Greca, Ariel Waisman, Lucia Natalia Moro, G Sevlever, S Miriuka, Carlos Luzzani

Scientific Reports 2021


Generation of a human induced pluripotent stem cell line from a familial Alzheimer´s disease PSEN1 T119I patient

 Isaja , María Soledad Rodríguez-Varela , Mariela Marazita , Sofía Mucci , Tatiana Itzcovich , Patricio Chrem-Méndez, Matías Niikado , Sofía Luján Ferriol-Laffouillere , Ricardo Allegri , Horacio Martinetto , Gustavo Emilio Sevlever , María Elida Scassa, Ezequiel Ignacio Surace , Leonardo Romorini

Stem Cell Research 2021


2020

Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism

Isaja L, Mucci S, Vera J, Rodríguez-Varela MS, Marazita M, Morris-Hanon O, Videla-Richardson GA, Sevlever GE, Scassa ME, Romorini L.

Sci Rep. 2020 

Generation of Myostatin Edited Horse Embryos Using CRISPR/Cas9 Technology and Somatic Cell Nuclear Transfer

Lucia Natalia Moro, Diego Luis Viale, Juan Ignacio Bastón, Victoria Arnold, Mariana Suvá, Elisabet Wiedenmann, Martín Olguín, Santiago Miriuka, Gabriel Vichera

Scientific Reports 2020


 PIWI-interacting RNAs are differentially expressed during cardiac differentiation of human pluripotent stem cells

Alejandro La Greca, María Agustina Scarafía, María Clara Hernández Cañás, Nelba Pérez, Sheila Castañeda, Carolina Colli, Alan Miqueas Möbbs, Natalia Lucía Santín Velazque, Gabriel Neiman, Ximena Garate, Cyntia Aban, Ariel Waisman, Lucía Natalia Moro, Gustavo Sevlever, Carlos Luzzani, Santiago Gabriel Miriuka

Plos One, 2020


Celldeath: a tool for simple detection of cell death in transmitted light microscopy images by visual deep learning analysis

Alejandro La Greca, Nelba Pérez, Paula Melania Milone, Sheila Castañeda, María Agustina Scarafía, Alan Miqueas Möbbs, Ariel Waisman, Lucía Natalia Moro, Gustavo Sevlever, Carlos Luzzani, Santiago Gabriel Miriuka
Biorxiv, 2020

2019

Deep Learning Neural Networks Highly Predict Very Early Onset of Pluripotent Stem Cell Differentiation

Ariel Waisman, Alejandro La Greca, Alan M Möbbs, María Agustina Scarafía, Natalia L Santín Velazque, Gabriel Neiman, Lucía N Moro, Carlos Luzzani, Gustavo E Sevlever, Alejandra S Guberman, Santiago G Miriuka

Stem Cell Reports, 2019


Human embryonic stem cells display a pronounced sensitivity to the cyclin dependent kinase inhibitor Roscovitine

Guillermo A. Videla-Richardson, Verónica A. Furmento, Carolina P. Garcia, Olivia Morris-Hanon, Gustavo E. Sevlever, Leonardo Romorini & María E. Scassa 

BMC Molecular and Cell Biology volume, 2019


Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation

Gabriel Neiman, María Agustina Scarafía, Alejandro La Greca, Natalia L Santín Velazque, Ximena Garate, Ariel Waisman, Alan M Möbbs, Tais Hanae Kasai-Brunswick, Fernanda Mesquita, Daiana Martire-Greco, Lucía N Moro, Carlos Luzzani, Adriana Bastos Carvalho, Gustavo E Sevlever, Antonio Campos de Carvalho, Alejandra S Guberman, Santiago G Miriuka

Scientific Reports, 2019


Palbociclib effectively halts proliferation but fails to induce senescence in patient-derived glioma stem cells

Olivia Morris-Hanon, Mariela Claudia Marazita, Leonardo Romorini, Luciana Isaja, Damián Darío Fernandez-Espinosa, Gustavo Emilio Sevlever, María Elida Scassa & Guillermo Agustín Videla-Richardson 

Molecular Neurobiology volume, 2019


2018

Regulation of cyclin E1 expression in human pluripotent stem cells and derived neural progeny

María Soledad Rodríguez Varela, Sofía Mucci, Guillermo Agustín Videla Richardson, Olivia Morris Hanon, Verónica Alejandra Furmento, Santiago Gabriel Miriuka, Gustavo Emilio Sevlever, María Elida Scassa, Leonardo Romorini

Cell Cycle, 2018


Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells

Ximena Garate, Alejandro La Greca, Gabriel Neiman, Carolina Blüguermann, Natalia Lucía Santín Velazque, Lucía Natalia Moro, Carlos Luzzani, Maria Elida Scassa, Gustavo Emilio Sevlever, Leonardo Romorini, Santiago Gabriel Miriuka

Scientific Reports, 2018


Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses.

Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G.

Stem Cells Cloning. 2018 


MicroRNA characterization in equine induced pluripotent stem cells

LN Moro, G Amin, V Furmento, A Waisman, G Neiman, A La Greca, NL Santin, C Luzzani, G Sevlever, G Vichera, SG Miriuka

Plos One, 2018


Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

Alejandro La Greca, Claudia Solari, Veronica Furmento, Antonella Lombardi, Maria Celeste Biani, Cyntia Aban, Lucia Moro, Marcela García, Alejandra Sonia Guberman, Gustavo Emilio Sevlever, Santiago Gabriel Miriuka, Carlos Luzzani

Experimental & molecular medicine, 2018


Leukemia Inhibitory Factor Increases Survival of Pluripotent Stem Cell-Derived Cardiomyocytes

Carolina Blüguermann, Leonardo Romorini, Denis Evseenko, Ximena Garate, Gabriel Neiman, Gustavo Emilio Sevlever, María Elida Scassa, Santiago Gabriel Miriuka

Journal of cardiovascular translational research, 2018


2017

 The cell cycle inhibitors p21cip1 and p27kip1 control proliferation but enhance DNA damage resistance of glioma stem cells

OliviaMorris-Hanon, Verónica Alejandra Furmento, María Soledad Rodríguez-Varela, SofíaMucci, Damián DaríoFernandez-Espinosa, Leonardo Romorini, Gustavo Emilio Sevlever, María Elida Scassa, Guillermo Agustín Videla-Richardson

Neoplasia, 2017


Pluripotent stem cells as a robust source of mesenchymal stem cells 

Carlos D Luzzani, Santiago G Miriuka

Stem Cell Reviews and Reports, 2017


2016

 In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.

Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G.

PLoS One. 2016 


AKT/GSK3β signaling pathway is critically involved in human pluripotent stem cell survival

Leonardo Romorini, Ximena Garate, Gabriel Neiman, Carlos Luzzani, Verónica Alejandra Furmento, Alejandra Sonia Guberman, Gustavo Emilio Sevlever, María Elida Scassa, Santiago Gabriel Miriuka

Scientific Reports, 2016


Human pluripotent stem cells and derived neuroprogenitors display differential degrees of susceptibility to BH3 mimetics ABT-263, WEHI-539 and ABT-199

Carolina Paola García, Guillermo Agustin Videla Richardson, Nicolás Alexis Dimopoulos, Damian Dario Fernandez Espinosa, Santiago Gabriel Miriuka, Gustavo Emilio Sevlever, Leonardo Romorini, María Elida Scassa

PloS one, 2016


Generation of iPSC line iPSC-FH2. 1 in hypoxic conditions from human foreskin fibroblasts

María Questa, Leonardo Romorini, Carolina Blüguermann, Claudia María Solari, Gabriel Neiman, Carlos Luzzani, María Élida Scassa, Gustavo Emilio Sevlever, Alejandra Sonia Guberman, Santiago Gabriel Miriuka

Stem cell research, 2016


Specific preferences in lineage choice and phenotypic plasticity of glioma stem cells under BMP4 and Noggin influence

Guillermo Agustín Videla Richardson, Carolina Paola Garcia, Alejandro Roisman, Irma Slavutsky, Damian Dario Fernandez Espinosa, Leonardo Romorini, Santiago Gabriel Miriuka, Naomi Arakaki, Horacio Martinetto, María Elida Scassa, Gustavo Emilio Sevlever

Brain Pathology, 2016


2015

 A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement

Carlos Luzzani, Gabriel Neiman, Ximena Garate, María Questa, Claudia Solari, Darío Fernandez Espinosa, Marcela García, Ana Lía Errecalde, Alejandra Guberman, María Elida Scassa, Gustavo Emilio Sevlever, Leonardo Romorini, Santiago Gabriel Miriuka

Stem cell research & therapy, 2015


2014 

Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells

Carolina Paola García, Guillermo Agustín Videla Richardson, Leonardo Romorini, Santiago Gabriel Miriuka, Gustavo Emilio Sevlever, María Elida Scassa

Stem cell research, 2014


2013

Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth

Leonardo Romorini, Diego Ariel Riva, Carolina Blüguermann, Guillermo Agustin Videla Richardson, Maria Elida Scassa, Gustavo Emilio Sevlever, Santiago Gabriel Miriuka

PLoS One, 2013


2012

Activation of apoptotic signalling events in human embryonic stem cells upon Coxsackievirus B3 infection

Leonardo Romorini, María E Scassa, Guillermo Videla Richardson, Carolina Blüguermann, Carolina Jaquenod de Giusti, María Questa, Damián D Fernandez Espinosa, Ricardo M Gómez, Gustavo E Sevlever, Santiago G Miriuka

Apoptosis, 2012


2011

  Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment

María E Scassa, Carolina Jaquenod de Giusti, María Questa, Gabriela Pretre, Guillermo A Videla Richardson, Carolina Bluguermann, Leonardo Romorini, María F Ferrer, Gustavo E Sevlever, Santiago G Miriuka, Ricardo M Gómez

Stem cell research, 2011


2010

Stem cell research in South America coming of age

Osvaldo L Podhajcer, Santiago Miriuka

Cell stem cell, 2010

 

Publicaciones en colaboración con otros grupos

2020

AHR is a Zika virus host factor and a candidate target for antiviral therapy

Giovannoni F, Bosch I, Polonio CM, Torti MF, Wheeler MA, Li Z, Romorini L, Rodriguez Varela MS, Rothhammer V, Barroso A, Tjon EC, Sanmarco LM, Takenaka MC, Modaresi SMS, Gutiérrez-Vázquez C, Zanluqui NG, Dos Santos NB, Munhoz CD, Wang Z, Damonte EB, Sherr D, Gehrke L, Peron JPS, Garcia CC, Quintana FJ.

Nat Neurosci. 2020 

2019

Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency

Ariel Waisman, Federico Sevlever, Martín Elías Costa, María Soledad Cosentino, Santiago G Miriuka, Alejandra C Ventura, Alejandra S Guberman

Scientific Reports, 2019


Kat6b Modulates Oct4 and Nanog Binding to Chromatin in Embryonic Stem Cells and Is Required for Efficient Neural Differentiation

María Soledad Cosentino, Camila Oses, Camila Vázquez Echegaray, Claudia Solari, Ariel Waisman, Yanina Álvarez, María Victoria Petrone, Marcos Francia, Marcelo Schultz, Gustavo Sevlever, Santiago Miriuka, Valeria Levi, Alejandra Guberman

Journal of Molecular Biology, 2019


 The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells

Claudia Solari, María Victoria Petrone, Ayelén Toro, Camila Vazquez Echegaray, María Soledad Cosentino, Ariel Waisman, Marcos Francia, Lino Barañao, Santiago Miriuka, Alejandra Guberman

BMC research notes, 2019


2018

Healthy Foals Produced Using Bone Marrow-Mesenchymal Stem Cells as Nuclear Donors in Horse Cloning

R Olivera, L Moro, R Jordan, C Luzzani, S Miriuka, G Vichera

Reproduction, Fertility and Development, 2018


Non-β-Blocking Carvedilol Analog, VK-II-86, Prevents Ouabain-Induced Cardiotoxicity

Luis A Gonano, Marisa Sepúlveda, Malena Morell, Tamara Toteff, María Florencia Racioppi, Elena Lascano, Jorge Negroni, María Julieta Fernández Ruocco, Emiliano Medei, Gabriel Neiman, Santiago G Miriuka, Thomas G Back, Wayne Chen SR, Alicia Mattiazzi, Martin Vila Petroff

Circulation Journal, 2018


Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

Ramiro Olivera, LN Moro, Roberto Jordan, Natalia Pallarols, Alejandro Guglielminetti, Carlos Luzzani, SG Miriuka, Gabriel Vichera

Stem cells and cloning: advances and applications, 2018


Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells

Claudia Solari, María Victoria Petrone, Camila Vazquez Echegaray, María Soledad Cosentino, Ariel Waisman, Marcos Francia, Lino Baranao, Santiago Miriuka, Alejandra Guberman

Mechanisms of development, 2018


2017

Investigating Molecular Mechanisms of Type 2 Long QT Syndrome With iPSC-Derived Cardiomyocytes

Fernanda C Mesquita, Gustavo Monnerat, Dayana S Araujo, Paulo C Arantes, Tais H Brunswick, Fernanda Gubert, Jorge Coutinho, Gabriel Neiman, Tamara Borgonovo, Isabela C Leitão, Danubia S dos Santos, Isadora M Vaz, Santiago Miriuka, Emiliano H Medei, Fernando E Cruz, Adriana B Carvalho, Antonio C Campos-de-Carvalho

Circulation, 2017


Inhibition of cell division and DNA replication impair mouse-naive pluripotency exit

Ariel Waisman, Camila Vazquez Echegaray, Claudia Solari, María Soledad Cosentino, Iain Martyn, Alessia Deglincerti, Mohammad Zeeshan Ozair, Albert Ruzo, Lino Barañao, Santiago Miriuka, Ali Brivanlou, Alejandra Guberman

Journal of molecular biology, 2017


Characterization of tunneling nanotubes in Wharton’s jelly mesenchymal stem cells. An intercellular exchange of components between neighboring cells

Viviana Sanchez, Nerina Villalba, Luciano Fiore, Carlos Luzzani, Santiago Miriuka, Alberto Boveris, Ricardo J Gelpi, Alicia Brusco, Juan José Poderoso

Stem Cell Reviews and Reports, 2017


2016

Cyclin Kinase-independent role of p21CDKN1A in the promotion of nascent DNA elongation in unstressed cells

Sabrina F Mansilla, Agustina P Bertolin, Valerie Bergoglio, Marie-Jeanne Pillaire, Marina A Gonzalez Besteiro, Carlos Luzzani, Santiago G Miriuka, Christophe Cazaux, Jean-Sebastien Hoffmann, Vanesa Gottifredi

Elife, 2016


In vitro and in vivo development of horse cloned embryos generated with iPSCs, mesenchymal stromal cells and fetal or adult fibroblasts as nuclear donors

Ramiro Olivera, Lucia Natalia Moro, Roberto Jordan, Carlos Luzzani, Santiago Miriuka, Martin Radrizzani, F Xavier Donadeu, Gabriel Vichera

PloS one, 2016


Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2

Claudia Solari, Camila Vazquez Echegaray, Carlos Luzzani, María Soledad Cosentino, Ariel Waisman, María Victoria Petrone, Marcos Francia, Alina Sassone, Jésica Canizo, Gustavo Sevlever, Lino Baranao, Santiago Miriuka, Alejandra Guberman

Biochemical and biophysical research communications, 2016


2015

Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells’ transcription factors

Claudia Solari, Camila Vazquez Echegaray, María Soledad Cosentino, María Victoria Petrone, Ariel Waisman, Carlos Luzzani, Marcos Francia, Emilly Villodre, Guido Lenz, Santiago Miriuka, Lino Barañao, Alejandra Guberman

PloS one, 2015


2014

Abnormal mitochondrial fusion–fission balance contributes to the progression of experimental sepsis

Analia Silvia Gonzalez, María Eugenia Elguero, P Finocchietto, Silvia Holod, Leonardo Romorini, Santiago Gabriel Miriuka, JG Peralta, Juan José Poderoso, Maria Cecilia Carreras

Free radical research, 2014


2013

Novel aspects of parenchymal–mesenchymal interactions: from cell types to molecules and beyond

Carolina Bluguermann, Ling Wu, Frank Petrigliano, David McAllister, Santiago Miriuka, Denis A Evseenko

Cell biochemistry and function, 2013


Human developmental chondrogenesis as a basis for engineering chondrocytes from pluripotent stem cells

Ling Wu, Carolina Bluguermann, Levon Kyupelyan, Brooke Latour, Stephanie Gonzalez, Saumya Shah, Zoran Galic, Sundi Ge, Yuhua Zhu, Frank A Petrigliano, Ali Nsair, Santiago G Miriuka, Xinmin Li, Karen M Lyons, Gay M Crooks, David R McAllister, Ben Van Handel, John S Adams, Denis Evseenko

Stem cell reports, 2013


EDA-containing fibronectin increases proliferation of embryonic stem cells

Noelia Losino, Ariel Waisman, Claudia Solari, Carlos Luzzani, Darío Fernández Espinosa, Alina Sassone, Andrés F Muro, Santiago Miriuka, Gustavo Sevlever, Lino Barañao, Alejandra Guberman

PloS one, 2013


2011

Modulation of chromatin modifying factors’ gene expression in embryonic and induced pluripotent stem cells

Carlos Luzzani, Claudia Solari, Noelia Losino, Waisman Ariel, Leonardo Romorini, Carolina Bluguermann, Gustavo Sevlever, Lino Barañao, Santiago Miriuka, Alejandra Guberman

Biochemical and biophysical research communications, 2011


Induced pluripotent stem cells’ self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium

Claudia Solari, Noelia Losino, Carlos Luzzani, Ariel Waisman, Carolina Bluguermann, Maria Questa, Gustavo Sevlever, Santiago Miriuka, Lino Barañao, Alejandra Guberman

Biochemical and biophysical research communications, 2011